DR4; RB X-100UG

Application

Research CategoryApoptosis & Cancer

Detect DR4 using this Anti-DR4 Antibody, C-terminus validated for use in WB.

Western blot: 1:500-1:1000

Whole cell lysate from HeLa cells can be used as a positive control and a 57 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: C-terminus

Polyclonal antibody raised against a peptide corresponding to amino acids 427-445 at C-terminus of human DR4 mature protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Specificity

Apoptosis, or programmed cell death, occurs during normal cellular diffferentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR4 (for death receptor 4) (Pan et al. 1997). The ligand for this novel cell death receptor has been identified and termed TRAIL, which is a new member in TRF family. Therefore, DR4 is also called TRAIL receptor-1 (TRAIL-R1) (Schneider et al. 1997). DR4 is expresssed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates TRAIL induced apoptosis and NF-kB activation in many tissues and cells.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.